党参多糖对巨噬细胞的诱导活化作用

doi:10.11843/j.issn.0366-6964.2017.04.022

Abstract

Abstract:

The RAW264.7 cells activation of Codonopsis pilosula polysaccharide (CPPS) was investigated through the detection of cell proliferation, TNF-α and IL-6 secretion and mRNA transcription, NF-кB signaling pathway activation. CPPS were obtained by water extraction and ethanol precipitation, then proteins were removed by Sevage method and the content was determined by Phenol-Sulfuric acid method. The proliferation of RAW264.7 cells incubated with different concentrations CPPS (2-fold serially diluted from 31.25 to 4 000 μg·mL^-1) was detected by MTT method. RAW264.7 cells incubated with various concentrations of CPPS (25, 50, 100, 150 and 200 μg·mL^-1), TNF-α and IL-6 secretion were measured using ELISA kits; The TNF-α and IL-6 mRNA transcription were evaluated by RT-PCR. As well, expression of NF-кB p65 and IкB-α was determined by Western blot. The content of CPPS was 83.97%; CPPS (31.25-250 μg·mL^-1) exerted proliferation effect on RAW264.7 cells, and the CPPS concentration of 125 μg·mL^-1 proliferated significantly (P<0.01). While the concentration of CPPS higher than 500 μg·mL^-1 inhibited the proliferation of RAW 264.7 cells. The CPPS (100 and 150 μg·mL^-1) could increase the secretion of TNF-α (P<0.01) and IL-6 (P<0.05), and significantly increased the transcription of TNF-α (P<0.001) and IL-6 (P<0.001). Moreover, CPPS concentration of 100 μg·mL^-1increased NF-кB p65 expression in nuclear (P<0.01) and decreased IкB-α expression in cytoplasm (P<0.001). CPPS can not only stimulate the RAW264.7 cells proliferation, possibly through activation of NF-кB signaling pathway to the secretion of TNF-α and IL-6 increased, which are involved in immune regulation.

CLC Number:

SHI Yi-nan, SUN Na, SUN Yao-gui, LI Hong-quan. Codonopsis pilosula Polysaccharide-induced Activation and Proliferation of Macrophage RAW264.7 cell[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(4): 777-784.

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Codonopsis pilosula Polysaccharide-induced Activation and Proliferation of Macrophage RAW264.7 cell

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